mouse pd 1 mpd 1  (R&D Systems)

Journal: bioRxiv

Article Title: Activity-based urinary biomarkers of response and resistance to checkpoint blockade immunotherapy

doi: 10.1101/2020.12.10.420265

Figure Lengend Snippet: a , αPD1-GzmB sensor conjugates (αPD1-GS) consist of αPD1 therapeutic antibody decorated with reporter-labeled GzmB peptide substrates (GS; AA sequence: IEFDSG). b , ELISA assays comparing binding affinity of αPD1-GS with unconjugated αPD1 using the mouse αPD1 clone 8H3 (log(agonist) vs. normalized response fitting function, n = 3). c , ELISA assays comparing binding affinity of αPD1-GS with unconjugated αPD1 using the rat αPD1 clone 29F.1A12 (log(agonist) vs. normalized response fitting function, n = 3). d , Representative flow cytometry histogram showing PD-1 expression of CD8+ TILs isolated from MC38 tumors. The same sample was divided and stained with either αPD1-GS, αPD1, or IgG1 isotype control. e , Quantified plot of PD-1 expression showing the median fluorescence intensity (MFI) of samples stained with either αPD1-GS, αPD1, or IgG1 isotype control (one-way ANOVA with Tukey’s post-test and correction for multiple comparisons, ns = not significant, n = 10). f , Tumor growth curves of MC38 tumors treated with αPD1-GS, αPD1, or IgG1 isotype control (two-way ANOVA with Tukey’s post-test and correction for multiple comparisons, ****P < 0.0001, n = 6).

Article Snippet: Binding of αPD1 conjugates to recombinant PD1 ligand was quantified using an ELISA assay developed in house, in which a high protein binding plate was coated with 1 ug/mL of recombinant Mouse PD-1 Protein (R&D, 9047-PD-100).

Techniques: Labeling, Sequencing, Enzyme-linked Immunosorbent Assay, Binding Assay, Flow Cytometry, Expressing, Isolation, Staining, Fluorescence